DMEM, Low Glucose, with Sodium Pyruvate, with L-Glutamine is one of the most widely used modifications of Eagle's medium.

DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins.Additionally, the formulation also includes glycine, serine, and ferric nitrate.

The culture medium has been supplemented L-Alanyl-L-Glutamine in place of  L-Glutamine.

Compared with Glutamine, L-Alanyl-L-Glutamine is much more stable than L-Glutamine, releases less ammonia in culture, and improves cell viability and growth.

The culture medium was originally used to culture embryonic mouse cells.

Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific to different cell lines.