Exosomes Research Solution
Exosomes are a type of extracellular vesicle (EV) with a diameter of approximately 30–150 nm. They contain nucleic acids, proteins, lipids, and other bioactive molecules. Almost all cell types can produce and release extracellular vesicles. Exosomes naturally occur in body fluids—including blood, saliva, milk, and urine—and can reach distant cells and tissues via the circulatory system, exerting intercellular regulatory functions. Exosomes hold great potential in basic research, translational applications, gene therapy, targeted drug delivery, and other fields related to cancer and other diseases. Consequently, exosomes have emerged as a major research hotspot in the life sciences.
XP Biomed provides a comprehensive solution for exosome research, including cell culture, exosome enrichment, isolation and purification, characterization, downstream analysis (e.g., RNA-omics, proteomics, and exosome tracking), and more.
Cell culture
• The sample sources for basic research on exosomes mainly include cell supernatant, serum, plasma, etc.
• When conducting cell culture to harvest cell supernatant samples, it is necessary to avoid interference from exogenous extracellular vesicles (such as serum) on the extracellular vesicles secreted by cells.
• The classic serum starvation culture method can obtain cell supernatant samples. However, after serum starvation, it may affect the quality of extracellular vesicles secreted by cells.
• VivaCell's extracellular vesicle free fetal bovine serum product meets the cGMP production requirements, with a serum extracellular vesicle removal efficiency of ≥ 99%. Cells can maintain the same growth rate and morphology as normal fetal bovine serum.
• When conducting cell culture to harvest cell supernatant samples, it is necessary to avoid interference from exogenous extracellular vesicles (such as serum) on the extracellular vesicles secreted by cells.
• The classic serum starvation culture method can obtain cell supernatant samples. However, after serum starvation, it may affect the quality of extracellular vesicles secreted by cells.
• VivaCell's extracellular vesicle free fetal bovine serum product meets the cGMP production requirements, with a serum extracellular vesicle removal efficiency of ≥ 99%. Cells can maintain the same growth rate and morphology as normal fetal bovine serum.
Exosome enrichment
• Extracellular vesicles are concentrated by a factor of 10–100X.
• No interference from endogenous extracellular vesicles presentpresents in serum.
• Reduction in apoptosis reduces cell debris.
• No serum starvation, more physiologic cell culture conditions.
• Continuous production over several months.
• No passaging of cells required.
• Closed, single use system.
• Manipulations such as heat shock can easily be applied uniformly to large numbers of cells.
• Continuous biomanufacturing process.
• Clinical scale under cGMP possible.
• Production at the gram scale possible using current technology.
• No interference from endogenous extracellular vesicles presentpresents in serum.
• Reduction in apoptosis reduces cell debris.
• No serum starvation, more physiologic cell culture conditions.
• Continuous production over several months.
• No passaging of cells required.
• Closed, single use system.
• Manipulations such as heat shock can easily be applied uniformly to large numbers of cells.
• Continuous biomanufacturing process.
• Clinical scale under cGMP possible.
• Production at the gram scale possible using current technology.
Exosome extraction
• MISEV 2018 proposed that different research objectives have different requirements for the purity of EVs, and selecting a suitable extracellular separation method is the most important.
• Differential ultracentrifugation is the most widely used method for separating and purifying EVs, and is regarded as the "gold standard" for EVs extraction. However, this method has no significant advantage in high-throughput extraction of exosomes.
• The "combination" method has become an important trend in current exosomes extraction, which can obtain high-quality exosomes for downstream analysis.
• Differential ultracentrifugation is the most widely used method for separating and purifying EVs, and is regarded as the "gold standard" for EVs extraction. However, this method has no significant advantage in high-throughput extraction of exosomes.
• The "combination" method has become an important trend in current exosomes extraction, which can obtain high-quality exosomes for downstream analysis.
Exosome identification
• Track and analyze the Brownian motion of each particle
• Sample processing is simpler, ensuring the original state of exosomes and faster detection speed
• The detection results include the size and concentration of extracellular vesicles,etc
• Sample processing is simpler, ensuring the original state of exosomes and faster detection speed
• The detection results include the size and concentration of extracellular vesicles,etc
Exosome tracing
Fluorescent labeling and tracing of extracellular vesicles can be used for functional research (such as cell co culture or animal in vivo imaging experiments).
The current mainstream labeling methods include the lipophilic dye PKH-67 (green)/PKH-26 (red), and the lipophilic carbonyl cyanin dye Dil/DiD/DiO/DiR.
Accurately quantify extracellular vesicles in unpurified samples using fluorescence time-resolved immunoassay.
The current mainstream labeling methods include the lipophilic dye PKH-67 (green)/PKH-26 (red), and the lipophilic carbonyl cyanin dye Dil/DiD/DiO/DiR.
Accurately quantify extracellular vesicles in unpurified samples using fluorescence time-resolved immunoassay.
Proteomic analysis
• Extracellular vesicles are rich in cell-derived proteins, lipids, DNA, and RNA (microRNA, mRNA, lncRNA, circRNA, etc.), which can be used for information exchange between cells.
• Exosomeomics analysis provides new targets for the diagnosis and immunotherapy of tumor diseases.
• Exosomeomics analysis provides new targets for the diagnosis and immunotherapy of tumor diseases.
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