MEM-α medium contains all 21 common amino acids and increases the concentration of some amino acids. In addition, it contains 5 vitamins, which is the most abundant version of MEM.

The culture medium has been supplemented with L-Alanyl-L-Glutamine in place of L-Glutamine.  Compared with L-Glutamine, L-Alanyl-L-Glutamine is much more stable, releases less ammonia in culture, and improves cell viability and growth.

The culture medium does not contain ascorbic acid, deoxyribonucleosides and ribonucleosides. 

The culture medium is produced to reach the super-low endotoxin (SLE) grade, with the endotoxin level ≤0.1 EU/mL as measured by the Limulus amebocyte lysate (LAL) method.