MEM contains higher concentrations of amino acids and is well suited for the growth needs of mammalian cells. This medium can also support the growth of suspension cells by reducing the calcium content.
The culture medium has been supplemented with L-Alanyl-L-Glutamine in place of L-Glutamine. Compared with L-Glutamine, L-Alanyl-L-Glutamine is much more stable, releases less ammonia in culture, and improves cell viability and growth.