IMDM is the first medium to be supplemented with HEPES (5958 mg/L) with better buffering capacity, making it ideal for high-density cell cultures that proliferate rapidly.
The culture medium has been supplemented with L-Alanyl-L-Glutamine in place of L-Glutamine. Compared with L-Glutamine, L-Alanyl-L-Glutamine is much more stable, releases less ammonia in culture, and improves cell viability and growth.