In conventional cell culture, serum is needed to maintain the proliferation ability of cells. Although serum can meet the needs of general cell culture, some specific background components still need to be removed in the experiment of optimizing nutritional components and labeling specific components for research. The common removal method is charcoal stripped .

Charcoal stripped of fetal bovine serum was used for in vitro culture, and eliminate background interference and thereby verify hormonal effects. The research included steroid receptor connection, steroid regulation of cell receptors, hormone secretion of various tissues and thyroid hormone function. In the production process, charcoal and dextran are used to remove hormones from serum.

Serum is usually used as an additive for cell growth, which has many functions and uses, such as: it is rich in macromolecular proteins and low molecular weight nutrients as an additional source of amino acids, carbohydrates and vitamins; Improve the buffering capacity of the medium; Can combine or neutralize toxic components; Reduce cell oxidative stress and reduce cell apoptosis; Provide growth-promoting factors to help cells adapt to the culture environment.

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