Alizarin Red S is a commonly used calcium-binding stain, which is widely used for the staining test of calcium-containing targets in biomedical samples. Free calcium ions can form red precipitates by chelating with alizarin red S; the fixed calcium will be directly dyed red.
Under the effect of osteogenic induction, mesenchymal stem cells (MSCs) will be gradually differentiated into osteoblasts, including the formation of calcified bone segments (calcium salt crystals) and the calcium secretion reaction of cells. Both can be dyed red by Alizarin Red S.
Use for staining of osteoblasts after osteogenic differentiation of mesenchymal stem cells, and recommend especially for staining verification after osteogenic differentiation using MSCgoTM Osteogenic XF medium.
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