

VIvacell Trypsin EDTA Solution A is designed not only to gently dissociate cells from almost any support substrates but also as well as from each other in order to actualize cell manipulation techniques in addition for other studies that require intact cell-surface proteins. Trypsin, as a solution, is available in a varied array of formulations with or without EDTA. EDTA is a chelator that binds calcium and magnesium ions that may otherwise inhibit the Trypsin activity which then hydrolyzes and gains access to the intercellular bonds being cell-cell and/or cell-substrate bonds.
Gentle digestion preserves high cell viability.
Digestion process can be terminated by PBS wash.
Trypsin is a pancreatic serine protease (proteolytic) enzyme with specificity for peptide bonds involving the carboxyl group of the basic amino acids, Arginine and Lysine. Trypsin often contains a crude mixture of lipases, nucleases, polysaccharides and proteases extracted from Porcine pancreas.
The trypsin digestion solution facilitates the transition of cultured cells from an adherent to a suspended state for in vitro applications.
Filter-sterilized and ready-to-use, this solution is suitable for digesting cultured cells or tissue samples without additional pretreatment.