Item Number：C3610
-
Specification & Item NumberC3610-0012（solution A,1.5 mL*4 ；solution B,1.5 mL*4）
-
Storage Conditions-20℃
-
Conditions CarriageBlue ice
-
Validity Period (Months)18
Product Introduction
Cell cultures can be synchronized by the addition of methotrexate (MTX), an inhibitor of thymidine biosynthesis which blocks cells in the S-phase (DNA synthesis) of the cell cycle. After 16-18 hours, most of the dividing cells in the culture are halted in the S-phase. If thymidine is added to the culture, the MTX block is released and the cells proceed synchronously to mitosis, at which point colcemid may be added. A very short colcemid treatment in conjuction with this technique may be used to produce high resolution analysis （extended prometaphase chromosomes） when small deletions or rearrangements are suspected.
Product Application
High resolution analysis is a special manipulation of the routine cytogenetics karyotyping procedure designed to provide a large number of mitotic figures in late prophase or prometaphase. At this stage of mitosis the chromosomes are longer and less condensed. With Cell synchronization kit the chromosomes will show greater level of band resolution not seen in routine analysis.
Suitable for Amniotic Fluid, Chorionic Villi, Bone Marrow, Lymphocytes and Hematopoietic cells.
Production And Quality System
Manufactured under ISO 13485 QMS and in compliance with applicable cGMP guidelines.
Download Product Related Materials
-
Online Message
-
-
Online consultation