产品介绍
Trypsin, an animal-derived product,is the most commonly used enzyme for harvesting cells in culture. Trypsin is apancreatic serine protease (proteolytic enzyme) with specificity for peptidebonds involving the carboxyl group of the two basic amino acids, arginine andlysine. Purified trypsin from porcine pancreas often contains a crude mixtureof lipases, nucleases, polysaccharides, and proteases.
VivaCell’s Trypsinis designed not only to gently dissociate cells from almost any supportsubstrates but also as well as from each other in order to actualize cellmanipulation techniques in addition to other studies that require intact cell surfaceproteins. As a solution, Trypsin, is available in a varied array offormulations with or without EDTA. EDTA is a chelator that binds calcium andmagnesium ions which may otherwise inhibit the trypsin activity. Trypsin is often the subculturing agent of choice for celldissociation of adherent cells, although the treatment may be cytotoxic ifprolonged. Over-trypsinization is a common cause of subculture problems. In a serum-free culture experiments, the trypsinreaction may be terminated by separating the cells from the solution viacentrifugation or by utilizing trypsin inhibitors such as Soybean TrypsinInhibitor (SBTI).
|
Cat. No. |
C3534-0100 |
C3538-0500 |
C3530-0100 |
C3532-0500 |
C3533-0500 |
|
Product Component |
Trypsin Solution A (0.25%), with Calcium and Magnesium, without Phenol Red |
Trypsin Solution B (0.25%), without Calcium and Magnesium, without Phenol Red |
Trypsin EDTA Solution A (0.25%), EDTA (0.02%), with Phenol Red |
Trypsin EDTA Solution B (0.25%), EDTA (0.05%), with Phenol Red |
Trypsin EDTA Solution C (0.05%), EDTA (0.02%), with Phenol Red |
|
Trypsin |
0.25% |
0.25% |
0.25% |
0.25% |
0.05% |
|
EDTA |
0.02% |
0.05% |
0.02% |
||
|
NaCl |
√ |
√ |
√ |
√ |
√ |
|
KCl |
√ |
√ |
√ |
√ |
√ |
|
Na2HPO4 |
√ |
√ |
√ |
||
|
KH2PO4 |
√ |
√ |
√ |
||
|
D-Glucose |
√ |
√ |
|||
|
Phenol Red |
√ |
√ |
√ |
||
|
NaHCO3 |
√ |
√ |
|||
|
CaCl2 · 2H2O |
√ |
||||
|
MgCl2 · 6H2O |
√ |
产品应用
Used for cell dissociation, routine cell culture passaging, and primary tissue dissociation
生产和质量体系
Manufactured under ISO 13485 QMS and in compliance with applicable cGMP guidelines.
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