PODS® Human BDNF (Brain-derived neurotrophic factor, 脑源性神经营养因子)
PODS® Human BDNF
PODS® co-crystals | PODS® co-crystals |
CellGS Catalogue Code |
Product Name |
Pack Size |
PPH1-50 |
PODS Human BDNF |
50 million |
PPH1-250 |
PODS Human BDNF |
250 million |
PPH1-1000 |
PODS Human BDNF |
1 billion |
PODS 技术
将生长因子包埋进蛋白质晶体(多角体蛋白)中,多角体蛋白能够保护包埋的生长因子,并维持其稳定性与生物活性,进而持续释放到细胞培养系统中。
PODS BDNF 描述
PODS多角体蛋白与BDNF共结晶。BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)是一种神经生长因子,它与低亲和力神经生长因子受体(LNGFR)和原肌球蛋白受体激酶B (TrkB)结合,支持神经元生长和存活。海马区BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)的表达对长期记忆的储存和学习至关重要。BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)的某些蛋白结构域与NGF和另一种神经营养因子NT-3(神经营养因子-3)的结构域相同。人、小鼠、大鼠和猪BDNF(脑源性神经营养因子)是交叉反应的。
PODS BDNF 规格
PODS BDNF Product Details |
|
Length |
164 aa |
Molecular Weight |
37.6 kDa |
Structure |
Dimer |
Source |
Spodoptera frugiperda (Sf9) cell culture |
Accession Number |
P23560 |
Endotoxin Level |
<0.06 EU/ml as measured by gel clot LAL assay |
Formulation |
PODS® were lyophilized from a volatile solution |
Reconstitution |
PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting. PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8). |
Stability and Storage |
Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended. |
PODS BDNF 使用建议
PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.
PODS BDNF AA Sequence
MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGLMHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKGQLKQYFY ETKCNPMGYT KEGCRGIDKR HWNSQCRTTQ SYVRALTMDS KKRIGWRFIR IDTSCVCTLT IKRGR
PODS BDNF Alternative Names
Brain-derived neurotrophic factor, neurotrophin, abrineurin, 脑源性神经营养因子
PODS BDNF 文件资料
PPH1 Datasheet | PODS User Guide | PODS MSDS |
更多PODS 相关产品:PODS 生长因子
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货号: PPH1
品牌:Cell Guidance Systems 
描述:CellGS PODS® Human BDNF (Brain-derived neurotrophic factor, neurotrophin, abrineurin, 脑源性神经营养因子)
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