Exo-spin™ mini-HD纯化管柱 EX05

产品介绍：

EX05 Exo-spin™ 试剂盒包含mini-HD SEC纯化管柱（column）。

Mini-HD Exo-spin™色谱柱和树脂孔径

纯化管柱床体积为3.5ml，在柱顶部可以加150ul的液体。填充树脂的孔径大约为30nm，以实现高纯度的外泌体洗脱。小于30 nm的所有其他分子（例如蛋白质，脂质）将进入孔中并保留在管柱中。

产品数据：

【使用Exo-spin™ SEC纯化管柱，提取100ul人血清中外泌体，分组收集组分，并进行分析，利用Bradford 方法测定蛋白浓度（灰），利用NTA方法测定颗粒浓度（蓝）】

订购信息：

下游应用：建议和内容

RNA analysis:

• Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A and Tripodi M. (2016). The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Reports17(3):799-808.

• Brook AC, Jenkins RH, Clayton A, Kift-Morgan A, Baby AC, Shephard AP, Mariotti B, Cuff SM, Bazzoni F, Bowen T, Fraser DJ, and Eberl M. (2019). Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9(1):10136. 

Immunoassay:

• Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A and Tabi Z. (2017). Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1):1368823.

Exosome engineering for therapeutic cargo:

• Kojima R, Bojar D, Rizzi G, Hamri GC, El-Baba MD, Saxena P, Ausländer S, Tan KR and Fussenegger M. (2018). Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment. Nature communications 9: 1305.

Functional study:

• Piao YJ, Kim HS, Hwang EH, Woo J, Zhang M and Moon WK. (2017). Breast cancer cell-derived exosomes and macrophage polarization are associated with lymph node metastasis. Oncotarget 9(7): 7398–7410.

Proteomic (MS/MS analysis):

• E L Kavanagh, S Lindsay, M Halasz, L C Gubbins, K Weiner-Gorzel, M H Z Guang, A McGoldrick, E Collins, M Henry, A Blanco-Fernández, P O' Gorman, P Fitzpatrick, M J Higgins, P Dowling, and A McCann. (2017). Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis 6(10): e388.

Protein assay:

• Welton JL, Webber JP, Botos LA, Jones M and Clayton A. (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4:27269.

产品资料

• EX05 Exo-spin™ user guide.pdf