VitroGel® IKVAV High Concentration
Overview
VitroGel® IKVAV is a ready-to-use tunable hydrogel system modified with laminin-derived functional peptide (IKAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α‐1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth.
Mix & Match – 3D Cell Culture Your WAY!
VitroGel® IKVAV is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel® RGD, VitroGel® YIGSR, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the micro-environment for 3D cell culture and more!
VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
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Specifications
Contents | VitroGel® IKVAV High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
Hydrogel Formulation | Xeno-free tunable hydrogel modified with IKVAV peptide. HIGH CONCENTRATION |
Use | Support neuronal progenitor cells differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth |
Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
Operation | Room temperature |
Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
pH | Neutral |
Color | Transparent |
Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
Injectable | Injectable hydrogel |
Storage | Store at 2-8°C. Ships at ambient temperature |
Product Documentation
Data and References
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Figure 1. Rheological properties of VitroGel IKVAV with DMEM medium. A) The gel formation curve after mixing with DMEM medium. VitroGel IKVAV was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G'of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
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Figure 2. 3D culture of glioblastoma cells (SNB 75) in VitroGel IKVAV. Cells were cultured with 1:3 diluted VitroGel IKVAV according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
Mix & Match Hydrogel System – 3D Cell Culture Your Way
Mix VitroGel IKVAV with VitroGel MMP at different ratios
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Figure 3. 3D culture of glioblastoma cells (SNB 75) in the mixture of VitroGel IKVAV and VitroGel MMP. The mixing ratios of VitroGel IKVAV and VitroGel MMP are 3:1, 1:1 and 3:1 (V/V). Cells were cultured with a 1:3 diluted VitroGel mixture following the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
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