VitroGel® RGD High Concentration
Overview
*VitroGel® RGD-PLUS is now called VitroGel® RGD.
VitroGel® RGD is a ready-to-use tunable hydrogel system modified with a high concentration of RGD cell adhesive peptide, promoting the cell attachment and cell-matrix interactions during the 3D cell culture. Get high levels of integrin-binding activities to promote intercellular networks even after hydrogel dilution.
Mix & Match – 3D Cell Culture Your WAY!
VitroGel® RGD is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel® IKVAV, VitroGel® YISGR, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the micro-environment for 3D cell culture and more!
VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivostudies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
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Cannot find the functional hydrogel you want? Contact us for a customized hydrogel system.
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Need rheology data of VitroGel with your medium and mixture for publication? Check our data support service.
Specifications
Contents | VitroGel® RGD High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
Hydrogel Formulation | Xeno-free tunable hydrogel modified with RGD peptide. HIGH CONCENTRATION |
Use | Good for adhesion cells or cells requiring stronger cell-matrix interactions. |
Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
Operation | Room temperature |
Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
pH | Neutral |
Color | Transparent |
Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
Injectable | Injectable hydrogel |
Storage | Store at 2-8°C. Ships at ambient temperature |
Product Documentation
Data and References
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Figure 1.
Rheological properties of VitroGel RGD with DMEM medium. A) – C) The gel formation curve after mixing with DMEM (A), DMEM/F-12 (B), and RPMI (C) media. VitroGel RGD was diluted at 1:0,1:1, 1:2 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with media at 4:1 (v/v) ratio; D) – F) The gel strength after 24 hrs incubation in DMEM (D), DMEM/F-12 (E), and RPMI (F) media. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G'of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
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Figure 2.
3D culture of OP9 cells in both VitroGel 3D-RGD and VitroGel RGD-PLUS. (both hydrogels have been prepared at 1:3 dilution with VitroGel Dilution Solution (Type 1), The images were taken on day 2 and 7) VitroGel RGD-PLUS has better support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure.
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Figure 3.
3D view of OP9 cells growth in VitroGel 3D-RGD and VitroGel RGD-PLUS. Cell networking structure formed in VitroGel RGD-PLUS and only single cell suspension has been observed in VitroGel 3D-RGD.
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Figure 4.
3D culture of U87-MG cells in both VitroGel 3D-RGD and VitroGel RGD-PLUS. Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of both hydrogel. However, the morphologies of the 3D cell culture are very different. Unlike the spheroids structure in VitroGel 3D-RGD, U87-MG cells perform better cell networking structure and the cell morphology in VitroGel RGD-PLUS indicates a better cell-cell and cell-matrix interaction.
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