VitroGel 3D成功应用案例
下表列出了已在VitroGel 3D系统中成功测试的细胞系。可根据不同的细胞系和培养基组分优化VitroGel 3D水凝胶系统,以得到更好的结果。推荐培养不同的细胞系时,都进行水凝胶浓度梯度测试。
Tables of successful cell types with Vitrogel 3D (TWG001)
CELL TYPES | APPLICATIONS | CULTURE MEDIUM | DILUTION |
---|---|---|---|
4T1 cells | 3D culture | RPMI 1640 with 10% FBS | 1:2 |
BL5 human beta cells | 2D and 3D culture | DMEM with 10%FBS | 1:3 |
CD8+ T cells | 3D culture | RMPI 1640 with 10% FBS | 1:3 |
HeLa cells | 3D culture | DMEM with 10%FBS | 1:3 |
Human Nthy-ori 3-1 cells | 3D culture | RPMI 1640 with 10% FBS | 1:3 |
KHOS cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
MDA-MB-231 cells | 3D culture | RPMI 1640 with 10% FBS | 1:2 |
Priess human lymphoblastoid cells | 2D and 3D culture | RPMI 1640 with 10% FBS | 1:3 |
Red Blood Cells | 3D culture | Alsever's solution | 1:1 to 1:3 |
T47D cells | 3D culture | RPMI 1640 with 10% FBS | 1:2 |
U2OS cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
Tables of successful cell types with Vitrogel 3D-RGD (TWG002)
CELL TYPES | APPLICATIONS | CULTURE MEDIUM | DILUTION |
---|---|---|---|
4T1 cells | 3D culture | RPMI 1640 with 10% FBS | 1:2 |
A549 cells | 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
Au565 cells | 3D culture | RPMI 1640 with 10% FBS | 1:3 |
Beta TC3 cells | 3D culture | DMEM with 10% FBS | 1:3 |
BT 474 cells | 3D culture | DMEM with 10% FBS | 1:3 |
DLD1 cells | 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
Fuji cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
H460 cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
HCT 116 cells | 2D and 3D culture | McCoys 5 with 10% FBS | 1:1 |
HCT-8 cells | 3D culture | RPMI 1640 with 10% FBS | 1:3 |
HEK 293 cells | 3D culture | DMEM with 10% FBS | 1:3 |
Hela cells | 2D and 3D culture | DMEM with 10% FBS | 1:3 |
HepG2 cells | 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
Human iPSCs | 2D and 3D culture | mTeSR1 | 1:3 |
Ins-1 Cells | 3D culture | RPMI 1640 with 10% FBS | 1:3 |
MCF-12A cells | 3D culture | DMEM/F-12 with 10% FBS | 1:3 |
MDA-MB-231 cells | 3D culture | L-15 medium with 10% FBS | 1:1 to 1:3 |
Melanoma cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
OVCAR-3 cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
Panc-1 cells | 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
SKB3 cells | 2D and 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
SYO-1 cells | 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
T47D cells | 3D culture | RPMI 1640 with 10% FBS | 1:3 |
UD 145 cells | 3D culture | EMEM with 10% FBS | 1:1 to 1:3 |
Tables of successful cell types with Vitrogel 3D RGD-PLUS (TWG003)
CELL TYPES | APPLICATIONS | CULTURE MEDIUM | DILUTION |
---|---|---|---|
MCF-7 cells | 2D and 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
MCF-12A cells | 3D culture | DMEM/F-12 with 10% FBS | 1:3 |
MDA-MB-231 cells | 3D culture | L-15 medium with 10% FBS | 1:1 to 1:3 |
OP9 cells | 2D and 3D culture | Alpha-MEM with 10% FBS | 1:3 |
Panc-1 cells | 2D and 3D culture | DMEM with 10% FBS | 1:1 to 1:3 |
SF 268 cells | 2D and 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
SF 295 cells | 2D and 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
SNB 75 cells | 2D and 3D culture | RPMI 1640 with 10% FBS | 1:1 to 1:3 |
U-87 MG cells | 2D and 3D culture | EMEM with 10% FBS | 1:1 to 1:3 |
U-251 MG cells | 2D and 3D culture | EMEM with 10% FBS | 1:1 to 1:3 |
VitroGel 3D为细胞创造一个功能性和优化的环境,让细胞感觉在家一样。从2D包被,3D细胞培养到动物注射,VitroGel 3D让利用相同系统进行体内体外研究成为可能。用户使用VitroGel 3D系统主要用于研究癌症和肿瘤细胞生物学,干细胞和组织工程,药物发现和毒性研究等方面。
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