VitroGel™3D 即用型水凝胶FAQ-Applications
Applications
Q Is the VitroGel compatible with drug/compound treatments?
A Yes, the VitroGel hydrogels are a great system to build 3D cell models for drug/compound screening. VitroGel is compatible for high-throughput screening with an auto liquid handling machine. Molecules can be mixed with the hydrogel or be added directly from the top of the hydrogel. This will allow an easy diffusion through the gel.
Q Can VitroGel perform transfection studies?
A Yes, VitroGel can be used to perform 3D transfection studies.
Q Can I use VitroGel to cover cells or tissue slides?
A Yes, VitroGel can be added directly on top of the cells or tissue slide.
Q Can I use VitroGel for in vivo studies?
A Yes, VitroGel can be injected before or after soft hydrogel formation for in vivo study.
Before the hydrogel formation: The VitroGel solution can be injected directly into the animal. It will become a hydrogel when it encounters the ionic compounds of the physiological environment.
After soft hydrogel formation: The VitroGel hydrogel has an advanced injectable property after soft hydrogel formation. Mixing the hydrogel solution and cell culture media/PBS at a proper ratio, the final hydrogel becomes injectable for in vivo studies. Using this method, cells or other chemical compounds can be mixed in the hydrogel before injection.
Q Can VitroGel be used for cell therapy?
A Yes, VitroGel is an excellent delivery system for cell therapy. Using VitroGel as an injectable delivery system, scientists can achieve better cell retention and higher cell viability for cell therapy.
Q What type of needles can you use for animal injection?
A We normally use 18-30 G needle size.
Q How can I use VitroGel for co-culture or sandwich culture (layer by layer)?
A The co-culture of two or more different cell types can be performed with the VitroGel system. Besides adding different cell types together with the hydrogel solution for co-culture, each cell type can also be mixed with the hydrogel solution and be added layer by layer. After the first layer of hydrogel become stable, carefully overlay the second layer of cells/hydrogel mixture on top of the first layer of cells/hydrogel.
Q Can DMSO be used with the VitroGel? If so, what is the maximum amount of DMSO that can be mixed with the hydrogel?
A Yes, DMSO can be used with VitroGel. For the maximum amount of DMSO, we suggest no more than 50% of the volume.
Q Can VitroGel be used for cryopreservation?
A Yes, VitroGel can be used for cryopreservation. It can help to promote cell viability.
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